Mfg Part Number
Ab02635-15.0
Vector Labs Ab02635-15.0 Anti-ZIKV E domain [A9E], Human IgM, lambda (50 μg)
Quick Overview
This antibody was generated using 6XL method (Kwakkenbos et al., 2010). Memory B cells were isolated from a subject previously infected with ZIKV. After identification of ZIKV-specific monoclonal cultures, genetic material of the variable domains was isolated and recombinant IgG1 lambda antibody forms were grafted with the sequences.
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| Product Class: | Purified |
|---|---|
| Clone ID: | A9E |
| Synonyms: | Zika envelope protein ; ZIKV; Zika virus E domain; E domain |
| Amount: | 50 μg |
| Application Codes Clone: | neutralizing; WB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds to ZIKV (probably its epitope spans part of EDIII and EDIII/EDI linker) and does not cross-react with DENV4, which was tested as a representative DENV serotype. Its epitope is distinct from the G9E anti-ZIKV antibody. |
| Application Notes: | This antibody has very strong neutralizing properties against Asian and African lineages of ZIKV (Collins et al., 2019). Furthermore, this antibody was effective in preventing Zika infection when administered from one day before the infections in mice (Collins et al., 2019). Therefore, A9E is recommended in development of diagnostic assays which differentiate ZIKV from other flaviviruses (it does not crossreact with DENV) and in the development of therapeutics and vaccines against ZIKV. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |