Mfg Part Number
Ab02644-23.0
Vector Labs Ab02644-23.0 Anti-Fibronectin [L19 (Radretumab)], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated using phage display from a human antibody library.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | L19 (Radretumab) |
| Synonyms: | FN; Cold-insoluble globulin; CIG; EDB(+) FN; ED-B domain of fibronectin; Extra domain-B containing fibronectin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC; RIA; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human fibronectin; it binds an epitope within the ED-B domain. Since there is a 100% homology between the human and murine ED-B domain, this antibody cross-reacts with mouse fibronectin. |
| Application Notes: | The original version of this antibody (human scFv) was determined to bind human fibronectin at the ED-B domain with a Kd of 54 pM using SPR. Furthermore, the antibody forms a stable complex with the fluorescently labeled ED-B and exhibits a long half-life when complexed with it (Pini et al., 1998; PMID: 9705314). This antibody was used for ELISA on biotinylated ED-B. Furthermore, it was used for a radioimmunoassay conducted on mice with subcutaneously implanted murine F9 teratocarcinoma, and for immunohistochemistry and Immunofluorescence performed on the corneas of New Zealand white rabbits (US8097254). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |