Mfg Part Number
Ab02651-10.0
Vector Labs Ab02651-10.0 Anti-CD4 [5A8], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing Balb/c mice with CHO cells expresssing full-length human CD4 on the cell surface.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5A8 |
| Synonyms: | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
| Amount: | 200 μg |
| Application Codes Clone: | RIA; WB; ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for nonreduced CD4. Human V1 residues (through amino acid 83) are not required for efficient 5A8 binding. human residues of the rod-like B strand which connects V1 and V2 (AA83 -AA105) significantly influence 5A8 binding. human CD4 V2 residues AA105 -AA131 (strands B and C of V2) are absolutely required for 5A8 binding. |
| Application Notes: | This antibody was used for a radioimmunoassay on human rsCD4 (US5871732). It was tested whether this antibody can block HIV-induced syncytia formation (US5871732) (Burkly et al, 1992; pmid:1380539). Western blot was used on different epitopes of CD4 using this antibody (US5871732). Immunohistochemistry was performed on human tissue with a humanized version of this antibody (Boon et al, 2002; pmid:11893418). ELISA with this antibody was used to detect bound sCD4 (Moore et al, 1992; pmid:1378510). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |