Mfg Part Number
Ab02667-23.0
Vector Labs Ab02667-23.0 Anti-14G2a [4B5], Rabbit IgG, lambda (200 μg)
Quick Overview
The original human version of this antibody was generated by treating humans with the 14G2a antibody then MNCs were harvested and hybridized with the murine myeloma cell line P3X.Ag8.653.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 4B5 |
| Synonyms: | antibody 14G2a; ganglioside; ganglioside 2a |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; funtional assay; RIA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for 14G2a. 14G2a is a mouse IgG2a antibody specific for human GD2. |
| Application Notes: | To determine the functionality of the mouse version of this antibody, an ELISA was performed to test if this antibody was able to bind to antigen 14G2A. Furthermore, the antibody was also tested in a western blot on antigen 14G2A (EP1000082A2). A radioimmunoassay was performed to screen for anti-14G2a activity of the human version of this antibody. To assess the ability of the human version of this antibody to mediate a cellular immune response. Rabbits were immunized with 4B5. Then, irradiated Mel-21 cells were implanted. The result was induration at the site of the irradiated cells (Saleh et al., 1993; PMID: 8376782). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |