Vector Labs Ab02671-10.3 Anti-HLA-A2 [BB7.2], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: BB7.2
Heavy Chain Modification: Fc Silent™
Synonyms: HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28
Amount: 200 μg
Application Codes Clone: ELISA; WB; FC; IF; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.
Application Notes: The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).

Product Class:	Purified
Clone ID:	BB7.2
Heavy Chain Modification:	Fc Silent™
Synonyms:	HLA-A; Human leukocyte antigen A; HLA class I histocompatibility antigen; A-2 alpha chain; MHC class I antigen A-2 HLA-A; HLA class I histocompatibility antigen A-28 alpha chain; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A-28
Amount:	200 μg
Application Codes Clone:	ELISA; WB; FC; IF; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.
Application Notes:	The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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