Mfg Part Number
Ab02673-2.3
Vector Labs Ab02673-2.3 Anti-Yellow Fever Virus [2D12], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
The original mouse version of this antibody was raised by immunizing female Blab/c mice with the 17D YF vaccine.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2D12 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | YFV; protein E; 17D; 17D YFV; Envelope protein E |
| Amount: | 200 μg |
| Application Codes Clone: | RIA; FC; IF; ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for protein E of the 17D yellow fever virus. Protein E binds to host cell surface receptor and mediates fusion between viral and cellular membranes. |
| Application Notes: | To characterize the mouse version of this antibody a radioimmunoassay with this antibody was performed on Vero cells infected with 17D YF. Further the presence of this antibody was confirmed by a 90% plaque reduction neutralization test in Vero cells (Schlesinger et al, 1983; pmid:6187129). To develop a diagnostic test to determine the presence of yellow fever virus in the blood of a patient. An ELISA was performed on the 17D YFV antigen using the mouse version of this antibody (Monath and Nystrom, 1984; pmid:6364854). To define the process of YFV entry into target cell. Flow cytometry was preformed using the mouse version of this antibody on cells infected with YFV. (Fernandez-Garcia et al, 2016; pmid:26861019). Type specific and cross reactive determinants were identified using immunofluorescence on yellow fever viruses using the mouse version of this antibody (Monath et al, 1984; pmid:6206738). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |