Mfg Part Number
Ab02674-23.0
Vector Labs Ab02674-23.0 Anti-GXM [18B7], Rabbit IgG, kappa (200 μg)
Quick Overview
The original mouse version of this antibody was raised by infecting balb/c mice with C. neoformans and then immunizing them with glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT).
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| Product Class: | Purified |
|---|---|
| Clone ID: | 18B7 |
| Synonyms: | Glucuronoxylomannan; Cryptococcus neoformans capsular polysaccharide; CNPS |
| Amount: | 200 μg |
| Application Codes Clone: | FC; ELISA; IHC; functional assay; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for GXM from C.neoformans. GXM is a polysacharide experssed on the surface of c.neoformans. |
| Application Notes: | To characterize the mouse version of this antibody an ELISA was performed on native and de-O-acetylated serotype A GMX using the mouse version of this antibody (Casadevall et al, 1992; pmid:1583327). Further immunofluorescence was performed on yeast cells using the mouse version of this antibody (Casadevell et al, 1998; pmid:9624491). Binding of the mouse version of this antibody to normal and C. neoformans-infected tissues was studied by immunohistochemistry (Casadevell et al, 1998; pmid:9624491). The murine macrophage-like cell line J774.16 was used to determine the ability of the mouse version of this antibody to opsonize C. neoformans (Casadevell et al, 1998; pmid:9624491). The effect of the murine version of this antibody on human PMN phagocytosis of C. neoformans was measured by a flow cytometry (Casadevell et al, 1998; pmid:9624491). C3 binding studies were done with formalin-killed cells of strain 388. The kinetics were studied both in the presence and absence of the murine version of this antibody (Casadevell et al, 1998; pmid:9624491). The ability of the mouse version of this antibody to reduce serum antigen in A/J mice was tested (Casadevell et al, 1998; pmid:9624491). The mouse version of this antibody has shown promise in a phase 1 trial testing patients with treated cryptococcal meningitis (Larsen et al, 2005; pmid:15728888). While developing a diagnostic test for Cryptococcal capsular antigens. The mouse version of this antibody was used for ELISA to test cross-reactivity with capsid antigens of other yeast species (Sathongdejwisit et al, 2021; pmid:33922698). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |