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Mfg Part Number

Ab02687-1.1

Vector Labs Ab02687-1.1 Anti-Human Cytokeratin 8 [1E8-C6-B4], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice against the C-terminus (amino acids 472-483) of human Cytokeratin 8.

$517.40

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Description

Product Class: Purified
Clone ID: 1E8-C6-B4
Synonyms: K8; Cytokeratin 8; CK 8; 1E8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8; 1E8
Amount: 200 μg
Application Codes Clone: WB, IP, IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody is specific for the C-terminus of cytokeratin 8, one member of a diverse group of proteins called intermediate filament proteins. These proteins are often expressed in pairs. Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the cytoskeleton in simple epithelial cells. Cytokeratin 8 is present on the surface of intact hepatocytes, hepatocellular carcinoma cells, and breast cancer cells.
Application Notes: The original antibody detected CK 8 by western blot from BT20 breast cancer cells. Immunofluorescence was performed on BT20 cells using this antibody, showing the carboxyl terminus of CK 8 was exposed on the surfaces of breast cancer cells. The Fab fragment of the antibody was used for inhibition experiments on breast cancer cell lines BT20, MDA-MB-157, MCF-7 and purified CK 8. Results showed a decreased plasminogen binding in all the experiments. The Fab fragment was used to study the role of cell-surface CK 8 in promoting the activation of plasminogen by t-PA in MCF-7 cells; preincubation of MCF-7 cells with the Fab fragment caused a concentration-dependent decrease in the ability of the cell cultures to promote plasminogen activation (Hembrough et al, 1996; pmid:8810346).

Product Class:	Purified
Clone ID:	1E8-C6-B4
Synonyms:	K8; Cytokeratin 8; CK 8; 1E8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8; 1E8
Amount:	200 μg
Application Codes Clone:	WB, IP, IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody is specific for the C-terminus of cytokeratin 8, one member of a diverse group of proteins called intermediate filament proteins. These proteins are often expressed in pairs. Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the cytoskeleton in simple epithelial cells. Cytokeratin 8 is present on the surface of intact hepatocytes, hepatocellular carcinoma cells, and breast cancer cells.
Application Notes:	The original antibody detected CK 8 by western blot from BT20 breast cancer cells. Immunofluorescence was performed on BT20 cells using this antibody, showing the carboxyl terminus of CK 8 was exposed on the surfaces of breast cancer cells. The Fab fragment of the antibody was used for inhibition experiments on breast cancer cell lines BT20, MDA-MB-157, MCF-7 and purified CK 8. Results showed a decreased plasminogen binding in all the experiments. The Fab fragment was used to study the role of cell-surface CK 8 in promoting the activation of plasminogen by t-PA in MCF-7 cells; preincubation of MCF-7 cells with the Fab fragment caused a concentration-dependent decrease in the ability of the cell cultures to promote plasminogen activation (Hembrough et al, 1996; pmid:8810346).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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