Mfg Part Number
Ab02690-8.4

Vector Labs Ab02690-8.4 Anti-Nitric Oxide Synthase [3F7-B11-B5], Rat IgG2b, Fc Silent™, kappa (200 μg)

Quick Overview
The original antibody was generated by immunizing mice against purified, bovine cerebellum NOS.
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Product Class:Purified
Clone ID:3F7-B11-B5
Heavy Chain Modification:Fc Silent™
Synonyms:NOS; Nitric oxide synthase; NOS-3F7-B11-B5
Amount:200 μg
Application Codes Clone:WB, IHC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgM
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:The antibody recognizes mouse, rat and bovine NOS.
Application Notes:The antibody reacted with bovine cerebellum NOS as well as with structurally different NOSs from cultured bovine aortic endothelial cells and cultured RAW 264.7 macrophages in Western blot analysis. The reactivity of the antibody was confirmed by immunohistochemical staining of rat cerebellum, arterial endothelial cells, and cultured stimulated macrophages. The antibody showed immunoreactivity within the respiratory epithelium but not in the bronchial smooth muscle (Rengasamy et al, 1994; pmid:7528982). The antibody was used for immunohistochemistry study to investigate the neuronal nitric oxide synthase immunoreactivity in cats (Papantchev et al, 2006; pmid:17120106). The antibody was used for immunohistochemical study on human colon tissue sample (Amato et al, 2014; pmid:24443715).
More Information
Brand Vector Labs
UNSPSC 12161500