Mfg Part Number
Ab02704-1.1
Vector Labs Ab02704-1.1 Anti-E. coli LacI [9A5], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice against E.coli LacI.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 9A5 |
| Synonyms: | Lactose operon repressor |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody recognizes LacI and recombinant proteins containing the LacI tag. The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. These genes are repressed when lactose is not available to the cell, ensuring that the bacterium only invests energy in the production of machinery necessary for uptake and utilization of lactose when lactose is present. When lactose becomes available, it is converted into allolactose, which inhibits the lac repressor's DNA binding ability. Loss of DNA binding by the lac repressor is required for transcriptional activation of the operon. |
| Application Notes: | This antibody was used for immunohistochemistry on tissue sections from mice transgenic for the R transgene (muscle, skin, hippocampus, cerebellum) (Cronin et al, 2001; PMID: 11410531). The antibody was used to detect the lac repressor |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |