Mfg Part Number
Ab02714-23.0
Vector Labs Ab02714-23.0 Anti-hemagglutinin H1 [5589], Rabbit IgG, (200 μg)
Quick Overview
This antibody was raised by sequencing DNA from PMBCs from humans immunized with the split-virion (IIV) quadrivalent seasonal influenza vaccine Fluzone in 2016-2017.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5589 |
| Synonyms: | H1; haemagglutinin H1; H1-specific; #5889 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; functional assay; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the hemagglutinin protein of influenza. Hemagglutinin is a homotrimeric glycoprotein found on the surface of influenza viruses and is integral to its infectivity. |
| Application Notes: | ELISA on recombinant hemagglutinnin using the human version of this antibody has proven the specificity of this antibody. Further, hemagglutination inhibition assays (HAIs) were also performed to measure functional antibodies capable of inhibiting virus agglutination. For this assay the human antibody was mixed with the influenza virus to which erythrocytes were added. Then the HAI titer was determined by the reciprocal dilution of the last well that contained non-agglutinated RBCs. Finally, human PBMCs were stained using the human version of this antibody to be used for flow cytometry (Forgacs et al, 2021; pmid:33617543). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |