Mfg Part Number
Ab02716-10.3
Vector Labs Ab02716-10.3 Anti-hemagglutinin H3 [1664], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by sequencing DNA from PMBCs from humans immunized with the split-virion (IIV) quadrivalent seasonal influenza vaccine Fluzone in 2016-2017.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1664 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | H3; haemagglutinin H3; H3-specific; #1664 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; functional assay; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the hemagglutinin protein of influenza. Hemagglutinin is a homotrimeric glycoprotein found on the surface of influenza viruses and is integral to its infectivity. |
| Application Notes: | ELISA on recombinant hemagglutinnin has proven the specificity of the human version of this antibody. Further, hemagglutination inhibition assays (HAIs) were also performed to measure functional antibodies capable of inhibiting virus agglutination. For this assay the human antibody was mixed with the influenza virus to which erythrocytes were added. Then, the HAI titer was determined by the reciprocal dilution of the last well that contained non-agglutinated RBCs. Finally, human PBMCs were stained for flow cytometry using the human version of this antibody (Forgacs et al, 2021; pmid:33617543). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |