Vector Labs Ab02722-23.0 Anti-HPV16-L1 [H16.V5], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: H16.V5
Synonyms: L1; Major capsid protein L1
Amount: 200 μg
Application Codes Clone: ELISA; neutralize; IP; IF; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes a surface confirmation epitope on the intact HPV-16 L1 virus like particles. This antibody is known to recognize the FG and HI loops, which are immunodominant in the humoral response against the HPV16 major capsid protein.
Application Notes: The specificity of this antibody against intact and disrupted virus-like particles (VLPs) of HPV-6, HPV-16, HPV-11, and HPV-18 was determined using ELISA (PMID: 8874508). This antibody was used as a capture antibody to test human serum IgG reactive with HPV-16 capsids (PMID: 8896492). This antibody was also reported to block HPV-16 VLP-induced agglutination of mouse RBC (PMID: 8806551). This antibody is capable of neutralizing by inhibiting HPV-16 binding to cell surfaces (PMID: 9223527). This antibody was also used to characterize a major neutralizing epitope on the HPV-16 type virus (PMID: 10233949). Sequences at both ends of the long FG loop (amino acids 260 to 290) were required for H16.V5 reactivity (PMID: 14557648). H16.V5 is often used for the assessment of the integrity and antigenicity of VLPs in vaccine products (PMID: 17012876). This antibody, when conjugated with magnetic beads, can also be used to immunoprecipitate HPV16 VLP and PsV out of cell lysates (PMID: 22927980). The binding to the surfaces of CaSki cells was detected by indirect immunofluorescence staining with H16.V5 and flow cytometry. The ability of gradient-purified HPV16 L1 to bind to the surface of SiHa cells at 4°C was detected by flow cytometric staining with the H16.V5 monoclonal antibody (PMID: 15890912).

Product Class:	Purified
Clone ID:	H16.V5
Synonyms:	L1; Major capsid protein L1
Amount:	200 μg
Application Codes Clone:	ELISA; neutralize; IP; IF; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes a surface confirmation epitope on the intact HPV-16 L1 virus like particles. This antibody is known to recognize the FG and HI loops, which are immunodominant in the humoral response against the HPV16 major capsid protein.
Application Notes:	The specificity of this antibody against intact and disrupted virus-like particles (VLPs) of HPV-6, HPV-16, HPV-11, and HPV-18 was determined using ELISA (PMID: 8874508). This antibody was used as a capture antibody to test human serum IgG reactive with HPV-16 capsids (PMID: 8896492). This antibody was also reported to block HPV-16 VLP-induced agglutination of mouse RBC (PMID: 8806551). This antibody is capable of neutralizing by inhibiting HPV-16 binding to cell surfaces (PMID: 9223527). This antibody was also used to characterize a major neutralizing epitope on the HPV-16 type virus (PMID: 10233949). Sequences at both ends of the long FG loop (amino acids 260 to 290) were required for H16.V5 reactivity (PMID: 14557648). H16.V5 is often used for the assessment of the integrity and antigenicity of VLPs in vaccine products (PMID: 17012876). This antibody, when conjugated with magnetic beads, can also be used to immunoprecipitate HPV16 VLP and PsV out of cell lysates (PMID: 22927980). The binding to the surfaces of CaSki cells was detected by indirect immunofluorescence staining with H16.V5 and flow cytometry. The ability of gradient-purified HPV16 L1 to bind to the surface of SiHa cells at 4°C was detected by flow cytometric staining with the H16.V5 monoclonal antibody (PMID: 15890912).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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