Mfg Part Number
Ab02737-23.159
Vector Labs Ab02737-23.159 Anti-Gelsolin [GsnVHH 13], Rabbit IgG-Fc Fusion, (200 μg)
Quick Overview
The original antibody was generated by immunizing a llama subcutaneously with human recombinant gelsolin.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | GsnVHH 13 |
| Synonyms: | GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IP; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes Ca2+ activated gelsolin. This antibody specifically recognizies and epitope in the G4-G5 domain of gelsolin. |
| Application Notes: | Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds calcium activated gelsolin with a Kd of ~10 nM. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. (PMID:20140750). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |