Vector Labs Ab02738-16.159 Anti-Gelsolin [GsnVHH 11 (Nb11)], Human IgA-Fc Fusion, (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: GsnVHH 11 (Nb11)
Synonyms: GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin
Amount: 100 μg
Application Codes Clone: ELISA; IP; WB; IF; immunotherapy
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes gelsolin irrespective of calcium and completely blocks its interaction with G-actin. This antibody specifically recognizies and epitope in the G2-G3 domain of gelsolin.
Application Notes: Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds gelsolin with a Kd of ~5 nM irrespective of calcium and completely blocks its interaction with G-actin. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane rufﬂes of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. It was also reported that free gelsolin is targeted to the nucleus by nuclear localizationsequence (NLS)-GsnVHH 11 in HeLa cells (PMID:20140750). Hereditary gelsolin amyloidosis is an autosomal dominantly inherited amyloid disorder caused by a point mutation in the GSN gene resulting in disturbed calcium binding and improper folding of the second gelsolin domain (G2) and rendering the mutant plasma gelsolin susceptible to a proteolytic cascade. This antibody was also used as a molecular chaperone to protect mutant plasma gelsolin from furin proteolysis in the trans-Golgi network (PMID: 25601851). This antibody was also used in the study of structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant (PMID: 30625383).

Product Class:	Purified
Clone ID:	GsnVHH 11 (Nb11)
Synonyms:	GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin
Amount:	100 μg
Application Codes Clone:	ELISA; IP; WB; IF; immunotherapy
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes gelsolin irrespective of calcium and completely blocks its interaction with G-actin. This antibody specifically recognizies and epitope in the G2-G3 domain of gelsolin.
Application Notes:	Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds gelsolin with a Kd of ~5 nM irrespective of calcium and completely blocks its interaction with G-actin. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane rufﬂes of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. It was also reported that free gelsolin is targeted to the nucleus by nuclear localizationsequence (NLS)-GsnVHH 11 in HeLa cells (PMID:20140750). Hereditary gelsolin amyloidosis is an autosomal dominantly inherited amyloid disorder caused by a point mutation in the GSN gene resulting in disturbed calcium binding and improper folding of the second gelsolin domain (G2) and rendering the mutant plasma gelsolin susceptible to a proteolytic cascade. This antibody was also used as a molecular chaperone to protect mutant plasma gelsolin from furin proteolysis in the trans-Golgi network (PMID: 25601851). This antibody was also used in the study of structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant (PMID: 30625383).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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