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Mfg Part Number

Ab02740-10.0

Vector Labs Ab02740-10.0 Anti-IGF1R [10H5], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated from BALB/c mice hyperimmunized with human IGF-IR extracellular domain (amino acids 1-902) in Ribi adjuvant. Later on the humanized version was created by grafting the hypervariable regions onto human framework regions.

$517.40

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Description

Product Class: Purified
Clone ID: 10H5
Synonyms: CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor
Amount: 200 μg
Application Codes Clone: ELISA; FC; IP;
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the extracellular domain of the human and cynomolgus monkey insulin-like growth factor and effective blocks ligand (IGF-I and IGF-II)-receptor (IGF-IR) interaction. This antibody does not bind mouse or rodent IGF-1R.
Application Notes: The orginal humanized IgG1 version of this antibody binds human IGF-1R with an affinity of 1.1 nM in an ELISA. The binding specificity was also comfirmed using fluorescence activated cell sorting of IGF-IR-expressing MCF7 cells. This antibody was also used for the immunoprecipitaion of MCF7 cells. The original antibody inhibits IGF-IR-mediated signaling by blocking IGF-I and IGF-II binding and by inducing cell surface receptor downregulation via internalization and degradation, with the extracellular and intracellular domains of IGF-IR being differentially affected by the proteasomal and lysosomal inhibitors. In vitro, h10H5 exhibits antiproliferative effects on cancer cell lines. In vivo, h10H5 shows single-agent antitumor efficacy in human SK-N-AS neuroblastoma and SW527 breast cancer xenograft models and even greater efficacy in combination with the chemotherapeutic agent docetaxel or an anti–vascular endothelial growth factor antibody (PMID:18790743).

Product Class:	Purified
Clone ID:	10H5
Synonyms:	CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor
Amount:	200 μg
Application Codes Clone:	ELISA; FC; IP;
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the extracellular domain of the human and cynomolgus monkey insulin-like growth factor and effective blocks ligand (IGF-I and IGF-II)-receptor (IGF-IR) interaction. This antibody does not bind mouse or rodent IGF-1R.
Application Notes:	The orginal humanized IgG1 version of this antibody binds human IGF-1R with an affinity of 1.1 nM in an ELISA. The binding specificity was also comfirmed using fluorescence activated cell sorting of IGF-IR-expressing MCF7 cells. This antibody was also used for the immunoprecipitaion of MCF7 cells. The original antibody inhibits IGF-IR-mediated signaling by blocking IGF-I and IGF-II binding and by inducing cell surface receptor downregulation via internalization and degradation, with the extracellular and intracellular domains of IGF-IR being differentially affected by the proteasomal and lysosomal inhibitors. In vitro, h10H5 exhibits antiproliferative effects on cancer cell lines. In vivo, h10H5 shows single-agent antitumor efficacy in human SK-N-AS neuroblastoma and SW527 breast cancer xenograft models and even greater efficacy in combination with the chemotherapeutic agent docetaxel or an anti–vascular endothelial growth factor antibody (PMID:18790743).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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