Vector Labs Ab02743-16.0 Anti-Spike protein [4A8], Human IgA1, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 4A8
Synonyms: SARS CoV 2 S glycoprotein; COVID-19 Spike protein; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Amount: 50 μg
Application Codes Clone: ELISA; FC; functional assay; neutralizing
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for S1 and S-ECD of the SARS CoV2 spike protein. SARS‑CoV‑2 is a positive-sense single-stranded RNA virus[5] that is contagious in humans.
Application Notes: The binding profile of this antibody was determined by performing an ELISA on SARS-CoV-2 S, S1, S2 or RBD protein using the human version of this antibody. Furthermore a neutralization experiment on Vero-E6 cells infected with SARS CoV2 was done using the human IgG1 version of this antibody. The binding of the human version was also characterized using biolayer interferometry. A flow cytometric receptor binding inhibition assay was also performed on the human version of this antibody incubated in SARS-CoV-2S protein and then incubated with ACE2-293T cells. The complex of S-ECD and the human version of this antibody was characterized using cryo electromicroscopy (Chi et al, 2020; pmid:32571838).

Product Class:	Purified
Clone ID:	4A8
Synonyms:	SARS CoV 2 S glycoprotein; COVID-19 Spike protein; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Amount:	50 μg
Application Codes Clone:	ELISA; FC; functional assay; neutralizing
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for S1 and S-ECD of the SARS CoV2 spike protein. SARS‑CoV‑2 is a positive-sense single-stranded RNA virus[5] that is contagious in humans.
Application Notes:	The binding profile of this antibody was determined by performing an ELISA on SARS-CoV-2 S, S1, S2 or RBD protein using the human version of this antibody. Furthermore a neutralization experiment on Vero-E6 cells infected with SARS CoV2 was done using the human IgG1 version of this antibody. The binding of the human version was also characterized using biolayer interferometry. A flow cytometric receptor binding inhibition assay was also performed on the human version of this antibody incubated in SARS-CoV-2S protein and then incubated with ACE2-293T cells. The complex of S-ECD and the human version of this antibody was characterized using cryo electromicroscopy (Chi et al, 2020; pmid:32571838).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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