Mfg Part Number
Ab02745-10.0
Vector Labs Ab02745-10.0 Anti-HIV-1 GP120 [PGT124], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was isolated from culture supernatants of approximately 30,000 activated memory B cells from a clade A infected donor by using a high-throughput strategy for screening IgG against monomeric gp120 (JR-CSF) and gp41(HxB2 -Env) binding as well as neutralization activity against HIV-1(JR-CSF) and HIV-1(SF162).
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| Product Class: | Purified |
|---|---|
| Clone ID: | PGT124 |
| Synonyms: | gp120; SU; Glycoprotein 120; Env protein; HIV Env; HIV-1 envelope glycoprotein; Envelope glycoprotein gp160; Envelope glycoprotein gp120; Glycoprotein 120; |
| Amount: | 200 μg |
| Application Codes Clone: | neutralize; ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody needs the N332 glycan and the GDIR motif to bind to the HIV-1 envelope glycoprotein (JRCSF strain). |
| Application Notes: | This broadly neutralizing antibody is capable of neutralizing HIV-1 strains 92RW020, 94UG103, 92BR020, JR-CSF, IAVI C22, BG505 and SF162. ELISA was used to determine the EC50 value of the antibody (PMID:28883821). To characterize the exact binding of this antibody, ITC binding experiments were performed using the fab version of this antibody with gp120 monomer. Further PGT124 Fab in complex with BG505 SOSIP.664 gp140 produced in HEK 293S cells were analyzed by negative stain EM. Finally To identify the epitope of the fab version of this antibody, the antibody was co-crystallized in complex with a gp120 core containing a mini-V3 loop. This crystal was subsequently used for crystallography to determine the structure of the complex (Garces et al, 2014; pmid:25259921). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |