Mfg Part Number
Ab02750-3.0
Vector Labs Ab02750-3.0 Anti-Testosterone [3C4F5], Mouse IgG2b, kappa (200 μg)
Quick Overview
The original mouse version of this antibody was raised by immunizing mice with a testosterone-3-carboxymethyloxime-thyroglobulin conjugate.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 3C4F5 |
| Synonyms: | ChEBI: 17347 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody binds testosterone and shows reasonably low cross-reaction (~11.6%) with 5a-dihydrotestosterone, androstenedione and ~1% cross reaction with dehydroepiandrosteronesulphate (DHEAS). Testosterone is the primary sex hormone and anabolic steroid in males. |
| Application Notes: | The original mouse IgG2b version of this antibody binds testosterone with an affinity of 0.3 nM. The binding properties of the antibody was preliminarily characterized by a competitive enzyme-linked immunosorbent assay. The combined (44 + 28) mutant derived from this antibody showed less cross-reactivity towards DHEAS of 0.033%, 44-fold less than in the wild-type (Hemminki et al, 1998; pmid:9680193). The combined mutant is available on request. To elucidate the structure of the mouse version of this antibody bound to testosterone x-ray crystallography was performed (Valjakka et al, 2002; pmid:11707437). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |