Vector Labs Ab02757-3.0 Anti-Estrogen receptor [JS34/32], Mouse IgG2b, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: JS34/32
Synonyms: ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Amount: 200 μg
Application Codes Clone: IHC; IP; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for human, bovine and rat estrogen receptor. The different molecular forms of the estrogen receptor also showed no difference in binding affinity. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Application Notes: Aliquots of 16a- [ 125I]iodo17P-estradiol-labeled receptor were immunoprecipitated with the mouse IgG2b version of this antibody (Moncharmont et al, 1982; pmid:7159574). To assess the localization of estrogen receptor (ER) in rat brain, pituitary and uterus, immunohistochemistry was performed on the brain, pituitary and uterus of rats (Sar and Parikh, 1986; pmid:2422450). To test whether the role of estrogen in breast cancer is dependent on a modified expression of their receptor, immunohistochemistry was performed on samples of human breast cancer using the mouse version of this antibody (Fabris et al, 1987; pmid:3695478). Determination of antibody interaction with microsomal and cytosolic estrogen-binding proteins was carried out by monitoring the shift in sedimentation coefficient of estrogen-estrophile complexes that occurs when they are bound by the mouse version of this antibody. This was done to characterize estrogen-binding sites associated with the endoplasmic reticulum of rat uterus (Evans Jr and Muldoon, 1991; pmid:2020979). To demonstrate the existence of receptors for estradiol in rabbit articular cartilage and to measure their concentration and dissociation constants, immunoprecipitation on cell cultures from rabbit cartilage tissue was performed using the mouse version of this antibody (Dayani et al, 1988; pmid:3419165). Immunohistochemistry was performed on human breast cancer tissue using the mouse version of this antibody, to show that immunohistochemistry is a viable method to assay cancer tissue (Marchetti et al, 1987; pmid:2436766).

Product Class:	Purified
Clone ID:	JS34/32
Synonyms:	ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Amount:	200 μg
Application Codes Clone:	IHC; IP; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for human, bovine and rat estrogen receptor. The different molecular forms of the estrogen receptor also showed no difference in binding affinity. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Application Notes:	Aliquots of 16a- [ 125I]iodo17P-estradiol-labeled receptor were immunoprecipitated with the mouse IgG2b version of this antibody (Moncharmont et al, 1982; pmid:7159574). To assess the localization of estrogen receptor (ER) in rat brain, pituitary and uterus, immunohistochemistry was performed on the brain, pituitary and uterus of rats (Sar and Parikh, 1986; pmid:2422450). To test whether the role of estrogen in breast cancer is dependent on a modified expression of their receptor, immunohistochemistry was performed on samples of human breast cancer using the mouse version of this antibody (Fabris et al, 1987; pmid:3695478). Determination of antibody interaction with microsomal and cytosolic estrogen-binding proteins was carried out by monitoring the shift in sedimentation coefficient of estrogen-estrophile complexes that occurs when they are bound by the mouse version of this antibody. This was done to characterize estrogen-binding sites associated with the endoplasmic reticulum of rat uterus (Evans Jr and Muldoon, 1991; pmid:2020979). To demonstrate the existence of receptors for estradiol in rabbit articular cartilage and to measure their concentration and dissociation constants, immunoprecipitation on cell cultures from rabbit cartilage tissue was performed using the mouse version of this antibody (Dayani et al, 1988; pmid:3419165). Immunohistochemistry was performed on human breast cancer tissue using the mouse version of this antibody, to show that immunohistochemistry is a viable method to assay cancer tissue (Marchetti et al, 1987; pmid:2436766).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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