Mfg Part Number
Ab02763-10.3
Vector Labs Ab02763-10.3 Anti-Estradiol [D12], Human IgG1, Fc Silent™, lambda (200 μg)
Quick Overview
This antibody was raised by panning against large single chain Fv library derived from lymphoid tissues including tonsil, bone marrow and peripheral blood lymphocytes.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | D12 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | 17-beta-estradiol; E2; ethynyl estradiol; estradiol; oestradiol; ChEBI: 16469; MSP1-D12 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for estradiol. Estradiol is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles. |
| Application Notes: | The scFv version of this antibody binds estradiol with an affinity of 3.7nM in and ELISA. To assess its physical characteristics the binding affinity of this antibody was also measured using SPR on estradiol using the scFv version of this antibody (Vaughan et al, 1996; pmid:9630891). To test the binding of this antibody, an ELISA was performed on either thyroglobulinoestradiol, BSA-oestriol or BSA using the scFv version of this antibody. Further the rate of association and dissociation was determined using surface plasmon resonance. This was performed with estradiol as antigen and the scFv version of this antibody (Pope et al, 1996; pmid:9373313). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |