Vector Labs Ab02764-24.1 Anti-NK1.1 [PK-136], Goat IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: PK-136
Synonyms: CD161c; Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c; Ly-55c; NK1.1; NKR-P1.9; NKR-P1C; Natural killer cell surface protein P1-40; NKR-P1 40; PK136
Amount: 200 μg
Application Codes Clone: FC; FACS; in vivo NK cell depletion
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric goat antibody was made using the variable domain sequences of the original format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody reacts with murine NK1.1. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain. NK1.1 plays a stimulatory role on natural killer (NK) cells cytotoxicity. Its activation by cross-linking of the receptor induces Ca2+ mobilization and interferon-gamma production.
Application Notes: The antibody was employed for FACS analysis of CD4+8- thymocytes (Arase et al., 1993; PMID: 8419184). The antibody was used for detection of NK1.1 of spleen cells by flow cytometry (Christianson et al., 1996; PMID: 8806787). The antibody was further used for the detection of NK1.1 by flow cytometry analysis (Arase et al., 1992; PMID: 1378629 and Lee et al., 2018; PMID: 30333822 and Coquet et al., 2008; PMID: 18685112). The antibody was employed for in vivo studies, the efficacy of the antibody in depleting the animals of ΝΚ1.1+ or NK T cells was ascertained by flow cytometric analysis. The reduction was greater than 90% after 1 week of treatment (Nomizo et al., 2005; PMID: 16162272). Finally, the antibody was used for the depletion of NK1.1+ cells in euthymic and thymectomized young C57Bl/6 mice infected with T. cruzi. The depletion of NK1.1 cells caused an increase in the parasitemia in young euthymic mice, but produced no such effects in thymectomized young mice infected with T. cruzi (Tulahuen strain) (Cardillo et al., 2002; PMID: 11841697).

Product Class:	Purified
Clone ID:	PK-136
Synonyms:	CD161c; Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c; Ly-55c; NK1.1; NKR-P1.9; NKR-P1C; Natural killer cell surface protein P1-40; NKR-P1 40; PK136
Amount:	200 μg
Application Codes Clone:	FC; FACS; in vivo NK cell depletion
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric goat antibody was made using the variable domain sequences of the original format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody reacts with murine NK1.1. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain. NK1.1 plays a stimulatory role on natural killer (NK) cells cytotoxicity. Its activation by cross-linking of the receptor induces Ca2+ mobilization and interferon-gamma production.
Application Notes:	The antibody was employed for FACS analysis of CD4+8- thymocytes (Arase et al., 1993; PMID: 8419184). The antibody was used for detection of NK1.1 of spleen cells by flow cytometry (Christianson et al., 1996; PMID: 8806787). The antibody was further used for the detection of NK1.1 by flow cytometry analysis (Arase et al., 1992; PMID: 1378629 and Lee et al., 2018; PMID: 30333822 and Coquet et al., 2008; PMID: 18685112). The antibody was employed for in vivo studies, the efficacy of the antibody in depleting the animals of ΝΚ1.1+ or NK T cells was ascertained by flow cytometric analysis. The reduction was greater than 90% after 1 week of treatment (Nomizo et al., 2005; PMID: 16162272). Finally, the antibody was used for the depletion of NK1.1+ cells in euthymic and thymectomized young C57Bl/6 mice infected with T. cruzi. The depletion of NK1.1 cells caused an increase in the parasitemia in young euthymic mice, but produced no such effects in thymectomized young mice infected with T. cruzi (Tulahuen strain) (Cardillo et al., 2002; PMID: 11841697).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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