Mfg Part Number
Ab02767-23.0
Vector Labs Ab02767-23.0 Anti-HA tag [12CA5], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing balb/c mice with a 9 amino acid petide to influenza virus hemagglutinin.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 12CA5 |
| Synonyms: | Rho GTPase-activating protein 45; Arhgap45; Minor histocompatibility protein HA-1 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; IF; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the HA-tag. HA contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity. |
| Application Notes: | The mouse version of this antibody has shown the ability to immunoprecipitate HA bound to BAX from cell lysates (Cheng et al, 1996; pmid:8596636). HA tagged SIM1 was immoprecipitated using the mouse version of this antibody. Other proteins present that lacked the HA tag were not immunoprecipitated (Michaud et al, 2000; pmid:10640708). A western blot has detected HA bound to PI4P5K using the mouse version of this antibody (Shibasaki et al, 1997; pmid:9065410). The HOXC6 protein bound to a HA-tag was immunoprecipitated using the mouse version of this antibody (McCabe et al, 2008; pmid:18339881). Cells expressing MLS2 fused to the HA epitope were stained using the mouse version of this antibody. Further the MSL2-HA protein was immuoprecipitated using this antibody (Smith et al, 2000; pmid:10594033). The Pat1 gene tagged with HA can be immunoprecipitated from lysed yeast cells using the mouse version of this antibody. Furthermore, the mouse version of this antibody can detect pat1-HA in a western blot. The western blot was preformed to determine the effect of topoisomerase II activity on chromosome segregation (Wang et al, 1996; pmid:8972867). HA tagged proteins can be stained using the mouse version of this antibody. Furthermore, the SPOP protein bound to a HA tag is able to be detected by the mouse version of this antibody on a western blot. The western blot was preformed to identify a novel antigen found in serum from a sclerodoma patient (Nagai et al, 1997; pmid:9414087). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |