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Mfg Part Number

Ab02768-7.1

Vector Labs Ab02768-7.1 Anti-Heparan Sulphate Proteoglycan [A7L6], Rat IgG2a, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells.

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Description

Product Class: Purified
Clone ID: A7L6
Synonyms: Perlecan; Perlecan proteoglycan; Heparan sulfate proteoglycan 2; Schwartz-Jampel syndrome 1; Endorepellin; Basement membrane-specific heparan sulfate proteoglycan core protein; LG3 peptide
Amount: 200 μg
Application Codes Clone: IP; IHC; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognizes and precipitates perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. This macromolecule has the power to store and protect growth factors, and thus plays a major rol in the control of tumour growth.
Application Notes: This original IgG2a rat clone can be used to detect and analyze the presence of perlecan. The specificity towards this protein has been confirmed using immunoprecipitation (Couchman, Ljubimov, 1989; PMID: 2477673). The perlecan was confirmed to reside in the basement membrane by means of immunofluorescence using this antibody (Couchman, Ljubimov, 1989; PMID: 2477673). Immunohistochemical staining confirmed that this clone also cross-reacts with other mammalian creatures (Couchman, Ljubimov, 1989; PMID: 2477673). In addition, one group used western blotting and immunohistochemical staining to detect significant differences in weight and distribution of antibodies across heparan sulfate proteoglycans (Tingbø et al., 2006; PMID: 16459119).

Product Class:	Purified
Clone ID:	A7L6
Synonyms:	Perlecan; Perlecan proteoglycan; Heparan sulfate proteoglycan 2; Schwartz-Jampel syndrome 1; Endorepellin; Basement membrane-specific heparan sulfate proteoglycan core protein; LG3 peptide
Amount:	200 μg
Application Codes Clone:	IP; IHC; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognizes and precipitates perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. This macromolecule has the power to store and protect growth factors, and thus plays a major rol in the control of tumour growth.
Application Notes:	This original IgG2a rat clone can be used to detect and analyze the presence of perlecan. The specificity towards this protein has been confirmed using immunoprecipitation (Couchman, Ljubimov, 1989; PMID: 2477673). The perlecan was confirmed to reside in the basement membrane by means of immunofluorescence using this antibody (Couchman, Ljubimov, 1989; PMID: 2477673). Immunohistochemical staining confirmed that this clone also cross-reacts with other mammalian creatures (Couchman, Ljubimov, 1989; PMID: 2477673). In addition, one group used western blotting and immunohistochemical staining to detect significant differences in weight and distribution of antibodies across heparan sulfate proteoglycans (Tingbø et al., 2006; PMID: 16459119).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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