Mfg Part Number
Ab02770-10.0

Vector Labs Ab02770-10.0 Anti-Laminin [A5], Human IgG1, kappa (200 μg)

Quick Overview
The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells.
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Product Class:Purified
Clone ID:A5
Synonyms:S-LAM gamma; Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; S-laminin subunit gamma
Amount:200 μg
Application Codes Clone:IP; IHC; IF
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2a
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody recognizes and precipitates laminin, a macromolecule prevalent in the basement membrane responsible for the attachment of cells to each other and forming tissue in the embryonic development of an organism.
Application Notes:This original rat IgG2a clone can be used to detect and analyze the presence of laminin. The specificity towards this protein has been confirmed using immunoprecipitation (Ljubimov et al., 1986; PMID: 3522258). The location of the laminin was confirmed to be in the basment membrane by means of immunofluorescence using this antibody (Eriksdotter-Nielson et al., 1986; PMID: 3537540).
More Information
Brand Vector Labs
UNSPSC 12161500