Mfg Part Number
Ab02770-10.0
Vector Labs Ab02770-10.0 Anti-Laminin [A5], Human IgG1, kappa (200 μg)
Quick Overview
The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | A5 |
| Synonyms: | S-LAM gamma; Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; S-laminin subunit gamma |
| Amount: | 200 μg |
| Application Codes Clone: | IP; IHC; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes and precipitates laminin, a macromolecule prevalent in the basement membrane responsible for the attachment of cells to each other and forming tissue in the embryonic development of an organism. |
| Application Notes: | This original rat IgG2a clone can be used to detect and analyze the presence of laminin. The specificity towards this protein has been confirmed using immunoprecipitation (Ljubimov et al., 1986; PMID: 3522258). The location of the laminin was confirmed to be in the basment membrane by means of immunofluorescence using this antibody (Eriksdotter-Nielson et al., 1986; PMID: 3537540). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |