Mfg Part Number
Ab02776-23.0
Vector Labs Ab02776-23.0 Anti-p53 (a.a. 16-25) [X77], Rabbit IgG, kappa (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised by immunizing mice against xenopus p53
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| Product Class: | Purified |
|---|---|
| Clone ID: | X77 |
| Synonyms: | Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IP; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes the human p73 protein as well as the human and xenopus p53 protein. |
| Application Notes: | This original mouse IgG1 can be used to detect human p73 as well as human and xenopus p53. The reaction of this antibody with the two proteins was verified using immunoprecipitation and western blot (Le Bras et al., 2002; PMID: 11850851). The same group used indirect immunofluorescence to verify the specificity of the antibody (Le Bras et al., 2002; PMID: 11850851). Another group used the antibody with immunoprecipitation and immunoblotting to detect the accumulation of human p53 with increasing levels of calpastatin (Pariat et al., 1997; PMID: 9111352). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |