Mfg Part Number
Ab02777-23.0
Vector Labs Ab02777-23.0 Anti-Alkaline phosphatase [VI-AP (2D5/5B8)], Rabbit IgG, kappa (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised by immunizing mice against calf alkaline phosphatase.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | VI-AP (2D5/5B8) |
| Synonyms: | ALP |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IF; ICC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody recognizes the calf alkaline phosphatase enzyme, and does not cross-react with humans. |
| Application Notes: | This original mouse IgG1 antibody mostly gets used as a negative control antibody, as the name suggests. One group used the antibody with immunofluorescence to control for other factors that could provide cell lysis, and provide a better view on how effective their own antibodies were (Sugita et al., 1986; PMID: 3456339). Another group used this antibody as a negative control in an immunofluorescence assay measuring the expression of certain antigens on human T-cells (Pickl et al., 1996; PMID: 8707334). A final group used the antibody with immunocytochemistry and flow cytochemistry as negative control in their research about the oncological proliferation marker Ki-76 (Nies et al., 2018; PMID: 30176186) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |