Mfg Part Number
Ab02778-10.0
Vector Labs Ab02778-10.0 Anti-Myc [33], Human IgG1, kappa (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised by immunizing mice against the full-length human c-myc protein. The spleen cells of these mice were then fused with mouse myeloma.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 33 |
| Synonyms: | Transcription factor p64; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; C-33 |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IP; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes the human proto-oncogene c-myc, a growth factor which is overexpressed in cancer cells. |
| Application Notes: | This original mouse IgG1 can be used to detect the human myc-c growth factor. The specificity of this antibody has been confirmed using immunoprecipitation and western blotting (Zhang et al., 2008; PMID: 18438430). In addition, one group used a western blot assay to research the myc-c levels in cells at different stages of cell growth (Shichiri et al., 1993; PMID: 8494788). Another group used western blotting to visualize the amount of myc-c in different tumours (Marin et al., 1995; PMID: 7698223). A final group used immunohistochemistry to analyze the role myc-c plays in tumour development (Banerjee et al., 1996; PMID: 8631582). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |