Vector Labs Ab02787-23.0 Anti-Gastrin [XPA067.06], Rabbit IgG, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: XPA067.06
Synonyms: Gastrin component III; Gastrin-17; G17
Amount: 200 μg
Application Codes Clone: functional assay; FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for gastrin 17. Gastrin is a peptide hormone that stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. It has also been implicated in the development or maintenance of various types of cancer, such as pancreatic and stomach carcinoma.
Application Notes: To determine the efficacy of gastrin neutralization by these antibodies in vitro, a flow cytometric measurement of intracellular calcium flux was performed using the human IgG1 version of this antibody on a CCK2R overexpressing cell line (AGS-TR). This antibody showed a 70%-80% neutralization in Ca flux in cells stimulated with gastrin (US8278421) (Hsu et al, 2008; pmid:18589401). Further the binding affinity of the human version of this antibody was determined using surface plasmon resonance and affinity constants were calculated from the kinetic rate constants for antibody binding to biotinylated peptide immobilized on a Streptavidin sensor chip (US8278421) (Hsu et al, 2008; pmid:18589401). The human version of this antibody has shown the ability to increase the stomach pH of mice to pH 4 in combination with human gastrin and famotidine. The pH with only human gastrin and famotidine was pH 2.5 (US8278421) (Hsu et al, 2008; pmid:18589401). Screening of gastrin positive antibody clones was done using ELISA (Hsu et al, 2008; pmid:18589401). Inhibition of gastrin activity has potential for therapeutic use as a suppressor of acid secretion as well as an inhibitor of gastrin-responsive tumors (Hsu et al, 2008; pmid:18589401).

Product Class:	Purified
Clone ID:	XPA067.06
Synonyms:	Gastrin component III; Gastrin-17; G17
Amount:	200 μg
Application Codes Clone:	functional assay; FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for gastrin 17. Gastrin is a peptide hormone that stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. It has also been implicated in the development or maintenance of various types of cancer, such as pancreatic and stomach carcinoma.
Application Notes:	To determine the efficacy of gastrin neutralization by these antibodies in vitro, a flow cytometric measurement of intracellular calcium flux was performed using the human IgG1 version of this antibody on a CCK2R overexpressing cell line (AGS-TR). This antibody showed a 70%-80% neutralization in Ca flux in cells stimulated with gastrin (US8278421) (Hsu et al, 2008; pmid:18589401). Further the binding affinity of the human version of this antibody was determined using surface plasmon resonance and affinity constants were calculated from the kinetic rate constants for antibody binding to biotinylated peptide immobilized on a Streptavidin sensor chip (US8278421) (Hsu et al, 2008; pmid:18589401). The human version of this antibody has shown the ability to increase the stomach pH of mice to pH 4 in combination with human gastrin and famotidine. The pH with only human gastrin and famotidine was pH 2.5 (US8278421) (Hsu et al, 2008; pmid:18589401). Screening of gastrin positive antibody clones was done using ELISA (Hsu et al, 2008; pmid:18589401). Inhibition of gastrin activity has potential for therapeutic use as a suppressor of acid secretion as well as an inhibitor of gastrin-responsive tumors (Hsu et al, 2008; pmid:18589401).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews