Vector Labs Ab02792-10.0 Anti-F glycoprotein [12B2], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 12B2
Synonyms: Fusion glycoprotein F0; Protein F; F protein; NiV F; HeV F; NiV; HeV
Amount: 200 μg
Application Codes Clone: neutralize; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds a conformation specific epitope on the F glycoprotein of the Nipah and Hendra virus. The nipah and hendra virus are spread by bats.
Application Notes: This antibody is reported to broadly neutralize nipah and hendra virus by blocking membrane fusion. Biolayer interferometry was used to characterize binding of the 12B2 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 27.7 and 5.35 nM for attachment of Fab fragments of 12B2 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. A cryo electron microscopy was performed on samples of the Fab fragment of this antibody bound to the F glycoprotein of the nipah virus (Dang et al, 2021; pmid:33927387). To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG version of this antibody (Dang et al., 2021; PMID: 33927387).

Product Class:	Purified
Clone ID:	12B2
Synonyms:	Fusion glycoprotein F0; Protein F; F protein; NiV F; HeV F; NiV; HeV
Amount:	200 μg
Application Codes Clone:	neutralize; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds a conformation specific epitope on the F glycoprotein of the Nipah and Hendra virus. The nipah and hendra virus are spread by bats.
Application Notes:	This antibody is reported to broadly neutralize nipah and hendra virus by blocking membrane fusion. Biolayer interferometry was used to characterize binding of the 12B2 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 27.7 and 5.35 nM for attachment of Fab fragments of 12B2 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. A cryo electron microscopy was performed on samples of the Fab fragment of this antibody bound to the F glycoprotein of the nipah virus (Dang et al, 2021; pmid:33927387). To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG version of this antibody (Dang et al., 2021; PMID: 33927387).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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