Vector Labs Ab02802-23.0 Anti-Collagen type II [M2139], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: M2139
Synonyms: M2 139; CII; Col II; CollA; ACPA; COL2A1; citrullinated CII; Col C II; citrullinated proteins; antibodies against citrullinated proteins; Collagen alpha-1(II) chain; Alpha-1 type II collagen
Amount: 200 μg
Application Codes Clone: ELISA; IHC; Blocking; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for the J1 epitope (amino acids 551–564 of collagen type II (MPGERRGAAGIAGPK)).
Application Notes: Epitope localization of the original format of this antibody (mouse IgG2b) was conducted using ELISA (Schulte et al., 1998; PMID: 9430695). this antibody, in combination with CIIC1, was utilized to induce collagen type II (Col II) antibody-induced arthritis in vivo in mice (Nandakumar et al., 2003; PMID: 14578183). This combination has later been shown to be potently arthritogenic in vivo, affecting cartilage matrix integrity in cartilage explant cultures, impairing synthesis of new matrix, and disrupting collagen fibril formation in chondrocyte cultures (Croxford et al., 2013; PMID: 23239042). Moreover, this antibody was employed in a study to investigate whether mAb CIIF4, when given in combination with this antibody and CIIC1, can prevent cartilage damage in bovine cartilage explants in vitro (Croxford et al., 2010; PMID: 20662051). The original version of this antibody was utilized to examine the production of collagen II with IHC staining (Gharravi et al., 2016; PMID: 26254592) and in the immunohistochemical staining of Col II (Soetjahjo et al., 2018; PMID: n/a).

Product Class:	Purified
Clone ID:	M2139
Synonyms:	M2 139; CII; Col II; CollA; ACPA; COL2A1; citrullinated CII; Col C II; citrullinated proteins; antibodies against citrullinated proteins; Collagen alpha-1(II) chain; Alpha-1 type II collagen
Amount:	200 μg
Application Codes Clone:	ELISA; IHC; Blocking; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for the J1 epitope (amino acids 551–564 of collagen type II (MPGERRGAAGIAGPK)).
Application Notes:	Epitope localization of the original format of this antibody (mouse IgG2b) was conducted using ELISA (Schulte et al., 1998; PMID: 9430695). this antibody, in combination with CIIC1, was utilized to induce collagen type II (Col II) antibody-induced arthritis in vivo in mice (Nandakumar et al., 2003; PMID: 14578183). This combination has later been shown to be potently arthritogenic in vivo, affecting cartilage matrix integrity in cartilage explant cultures, impairing synthesis of new matrix, and disrupting collagen fibril formation in chondrocyte cultures (Croxford et al., 2013; PMID: 23239042). Moreover, this antibody was employed in a study to investigate whether mAb CIIF4, when given in combination with this antibody and CIIC1, can prevent cartilage damage in bovine cartilage explants in vitro (Croxford et al., 2010; PMID: 20662051). The original version of this antibody was utilized to examine the production of collagen II with IHC staining (Gharravi et al., 2016; PMID: 26254592) and in the immunohistochemical staining of Col II (Soetjahjo et al., 2018; PMID: n/a).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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