Mfg Part Number
Ab02807-10.0
Vector Labs Ab02807-10.0 Anti-HIV-1 GP120 [b12 (MT12)], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was isolated from a combinatorial Fab phage display library generated from a long term asymptomatic male infected with clade B strain of HIV-1. Later on the Fab antibody was converted to an IgG1 by cloning the variable regions into a vector for mammalian expression.
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| Product Class: | Purified |
|---|---|
| Clone ID: | b12 (MT12) |
| Synonyms: | Glycoprotein 120; SU; surface protein gp120; env polyprotein; envelope glycoprotein gp16 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; Neutralize; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds CD4 binding site of the HIV-1 GP120. |
| Application Notes: | Binding affinity of the Fab antibody and the IgG1 version towards HIV-1 gp120 was determined using ELISA (US5804440). This antibody is a potent neutralizer of HIV strains IIIB and MN. The IgG1 version of b12 antibody showed 50% neutralization titers of less than 40ng/ml for both IIIB and MN strain in a p24 reporter assay. b12 can neutralize about 75% of clade B strains of HIV-1 (those most common in North America), but it neutralizes less than 50% of other strains of HIV-1 found worldwide (PMID: 7973652). This antibody has also shown promise in complete protection against HIV-1 in hu-PBL-SCID mouse model by passive immunization. The in vivo protective dose against viral challenge was found to be 4.5-7mg/kg (PMID: 7662189). This antibody is also reported to neutralize HXBc2 HIV-1 strain (PMID: 7518527). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |