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Mfg Part Number

Ab02807-23.0

Vector Labs Ab02807-23.0 Anti-HIV-1 GP120 [b12 (MT12)], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated from a combinatorial Fab phage display library generated from a long term asymptomatic male infected with clade B strain of HIV-1. Later on the Fab antibody was converted to an IgG1 by cloning the variable regions into a vector for mammalian expression.

$517.40

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Description

Product Class: Purified
Clone ID: b12 (MT12)
Synonyms: Glycoprotein 120; SU; surface protein gp120; env polyprotein; envelope glycoprotein gp16
Amount: 200 μg
Application Codes Clone: ELISA; Neutralize; therapeutic
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds CD4 binding site of the HIV-1 GP120.
Application Notes: Binding affinity of the Fab antibody and the IgG1 version towards HIV-1 gp120 was determined using ELISA (US5804440). This antibody is a potent neutralizer of HIV strains IIIB and MN. The IgG1 version of b12 antibody showed 50% neutralization titers of less than 40ng/ml for both IIIB and MN strain in a p24 reporter assay. b12 can neutralize about 75% of clade B strains of HIV-1 (those most common in North America), but it neutralizes less than 50% of other strains of HIV-1 found worldwide (PMID: 7973652). This antibody has also shown promise in complete protection against HIV-1 in hu-PBL-SCID mouse model by passive immunization. The in vivo protective dose against viral challenge was found to be 4.5-7mg/kg (PMID: 7662189). This antibody is also reported to neutralize HXBc2 HIV-1 strain (PMID: 7518527).

Product Class:	Purified
Clone ID:	b12 (MT12)
Synonyms:	Glycoprotein 120; SU; surface protein gp120; env polyprotein; envelope glycoprotein gp16
Amount:	200 μg
Application Codes Clone:	ELISA; Neutralize; therapeutic
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds CD4 binding site of the HIV-1 GP120.
Application Notes:	Binding affinity of the Fab antibody and the IgG1 version towards HIV-1 gp120 was determined using ELISA (US5804440). This antibody is a potent neutralizer of HIV strains IIIB and MN. The IgG1 version of b12 antibody showed 50% neutralization titers of less than 40ng/ml for both IIIB and MN strain in a p24 reporter assay. b12 can neutralize about 75% of clade B strains of HIV-1 (those most common in North America), but it neutralizes less than 50% of other strains of HIV-1 found worldwide (PMID: 7973652). This antibody has also shown promise in complete protection against HIV-1 in hu-PBL-SCID mouse model by passive immunization. The in vivo protective dose against viral challenge was found to be 4.5-7mg/kg (PMID: 7662189). This antibody is also reported to neutralize HXBc2 HIV-1 strain (PMID: 7518527).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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