Mfg Part Number
Ab02808-23.0
Vector Labs Ab02808-23.0 Anti-Cholera enterotoxin B subunit [TE33], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with CTP3 which is a conserved loop comprising amino acid residues 50-64 on the surface of the B subunit. CTP3 is involved in saccharide binding to the receptor on epithelial cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TE33 |
| Synonyms: | ctxB; toxB; VC_1456; Cholera enterotoxin subunit B; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; RIA; western blot |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds the CTP3 loop of the B subunit of the cholera toxin. This antibody is not capable of binding cholera toxin in its native form or its wild type form. TE33 specifically recognizes a loop comprising the sequence 'VPGSQHID' and a beta-turn formed by the sequence 'VPGS'. The CTP3 peptide is involved in saccharide binding to the receptor on the epithelial cells. |
| Application Notes: | The original IgG1 antibody which was raised against the CTP3 peptide also cross reacts with cholera toxin in a solid phase ELISA. The binding of the original IgG1 antibody to cholera toxin and H-LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli was done using solid-phase RIA and western blot (PMID: 2450576). In solution, the original antibody has a thousandfold lower affinity for the cholera toxin as compared to that for CTP3 (PMID: 1379072). The binding of the original antibody to both CTP3 peptide and cholera toxin was assayed by ELISA, solid-phase radioimmunoassay and immunoblot assay. The antibody was incapable of binding cholera toxin in its native form when tested in a solution-phase assay system (PMID: 1993848). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |