Mfg Part Number
Ab02809-1.159
Vector Labs Ab02809-1.159 Anti-Cholera enterotoxin B subunit [A9 (LCTA9)], Mouse IgG1-Fc Fusion, (100 μg)
Quick Overview
The original antibody was isolated from a non-immune semisynthetic llama library constructed from blood samples (6 ml) collected from the left jugular vein of three research naïve mature llamas.
$692.90
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | A9 (LCTA9) |
| Synonyms: | ctxB; toxB; VC_1456; Cholera enterotoxin subunit B; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 17165813 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds the B subunit of the cholera toxin. |
| Application Notes: | The binding specificity of this antibody towards cholera toxin was checked using ELISA (PMID:17165813). SPR was used to determine the dissociation constant (Kd) of VHH A9; the Kd was determined to be 77 ± 5 nM (PMID: 23385744). It was reported that two point mutations (N27D and R71I) help stabilize A9 wild-type form which has a relatively low melting point (PMID: 31342597). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |