Vector Labs Ab02816-2.3-VXS Anti-PD-1 [29F.1A12], Mouse IgG2a, Fc Silent™, kappa (1 mg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: VivopureX
Clone ID: 29F.1A12
Heavy Chain Modification: Fc Silent™
Synonyms: CD279; Programmed cell death protein 1; Protein PD-1; mPD-1
Amount: 1 mg
Application Codes Clone: FC; WB; Blocking, IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS only.
Original Format: IgG2a
Storage Temperature: All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions. Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes an epitope on mouse PD-1.
Application Notes: This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested; the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35% of PD-L1 Ig or PD-L2 Ig binding at 10 ug/ml. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003; pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020; pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021; pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al.; pmid:33117602).

Product Class:	VivopureX
Clone ID:	29F.1A12
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD279; Programmed cell death protein 1; Protein PD-1; mPD-1
Amount:	1 mg
Application Codes Clone:	FC; WB; Blocking, IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only.
Original Format:	IgG2a
Storage Temperature:	All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions. Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes an epitope on mouse PD-1.
Application Notes:	This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested; the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35% of PD-L1 Ig or PD-L2 Ig binding at 10 ug/ml. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003; pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020; pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021; pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al.; pmid:33117602).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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