Mfg Part Number
Ab02818-10.3
Vector Labs Ab02818-10.3 Anti-Glycoprotein E2 [HC33.1], Human IgG1, Fc Silent™, lambda (200 μg)
Quick Overview
The original antibody was isolated from a yeast display library constructed from peripheral B cells from a donor infected with HCV genotype 2b who had a high serum antibody binding titer to aa 410 to 425 and >1:10,000 neutralizing titer against genotype 2a JFH1 HCVcc.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | HC33.1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | NS1; gp68; gp70; HCV glycoprotein E2; Genome polyprotein; Envelope glycoprotein E2; E2 glycoprotein; JFH-1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; neutralize; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognizes a linear epitope comprising residues 412 to 423 of HCV envelope glycoprotein E2. It specifically recognizes the antigenic domain E of the virus comprising amino acids 413, 418 and 420 (PMID: 29703495). This antibody is also capable of recognizing and neutralizing the virus bearing N417S mutation associated with a glycosylation shift from Asn-417 to Asn-415 that enables HCV to escape neutralization by antibodies such as HCV1 and AP33 (PMID: 25737449). The hepatitis C virus is the cause of hepatitis C and some cancers such as liver cancer (hepatocellular carcinoma, abbreviated HCC) and lymphomas in humans. |
| Application Notes: | This broadly neutralizing antibody successfully neutralized JFH1 2a HCVcc and HCVcc isolates from genotypes 1a, 4a, and 5a. This antibody was found inhibit the binding of E2 glycoprotein to CD81 in a dose dependent manner. The binding affinity of the scFv version of this antibody was determined by using surface plasmon resonance in a BIAcore 3000. The binding specificity of the antibody to native or denatured recombinant E2 was determined by ELISA. Epitope mapping of the antibody was also done using ELISA (PMID: 23097455). HC33.1 was also reported to neutralize virus bearing the E2 N417S and N417T adaptive mutations with the glycosylation shift to Asn-415 (PMID: 25737449). This antibody was used in a western blot analysis to identify HEK293 and Sf9 derived sE2 (PMID: 30651366). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |