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Mfg Part Number

Ab02821-10.3

Vector Labs Ab02821-10.3 Anti-Glioma surface antigen [BT32/A6], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by fusing peripheral B lymphocytes (PBL) obtained from four patients with astrocytic tumors with human myeloma-like cell line TM-H2-SP2 to create hybridomas.

$517.40

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Description

Product Class: Purified
Clone ID: BT32/A6
Heavy Chain Modification: Fc Silent™
Synonyms: SK-MG-1; Glioma cells
Amount: 200 μg
Application Codes Clone: ELISA; FC; immunoperoxidase staining
Shipping Temperature: Wet Ice
Origin Pub PMID: 16971398
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody (BT32/A6) recognizes a surface and cytoplasmic antigen present on a variety of human tumor cell lines including gliomas, melanomas, neuroblastomas, and a few sarcomas.
Application Notes: The binding specificity of the IgM version of this antibody for human glioma cell lines was tested using ELISA (PMID: 1545260). Further characterization of binding of the IgM version of this antibody to SK-MG-1 and other cultured human cell lines was done using flow cytometry (US5639863; PMID: 7861227). This antibody was also used for the immunoperoxidase staining of human tumor cells (US5639863). Point mutation were introduced to increase the solubility and thermal refolding efficiency the antibody (PMID: 16971398). This antibody was also used to identify the non-human cell lines, normal human tissues, tumors of central neuroepithelial origin which express the antigen it binds (PMID: 9266445).

Product Class:	Purified
Clone ID:	BT32/A6
Heavy Chain Modification:	Fc Silent™
Synonyms:	SK-MG-1; Glioma cells
Amount:	200 μg
Application Codes Clone:	ELISA; FC; immunoperoxidase staining
Shipping Temperature:	Wet Ice
Origin Pub PMID:	16971398
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody (BT32/A6) recognizes a surface and cytoplasmic antigen present on a variety of human tumor cell lines including gliomas, melanomas, neuroblastomas, and a few sarcomas.
Application Notes:	The binding specificity of the IgM version of this antibody for human glioma cell lines was tested using ELISA (PMID: 1545260). Further characterization of binding of the IgM version of this antibody to SK-MG-1 and other cultured human cell lines was done using flow cytometry (US5639863; PMID: 7861227). This antibody was also used for the immunoperoxidase staining of human tumor cells (US5639863). Point mutation were introduced to increase the solubility and thermal refolding efficiency the antibody (PMID: 16971398). This antibody was also used to identify the non-human cell lines, normal human tissues, tumors of central neuroepithelial origin which express the antigen it binds (PMID: 9266445).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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