Mfg Part Number
Ab02823-10.3
Vector Labs Ab02823-10.3 Anti-C3a-receptor [2A1], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with second extracellular loop of the C3aR (amino acid residues 173–332) fused to glutathione-S-transferase (GST). A combinatorial phage library was generated expressing scFv antibodies and panned against second extracellular loop of the C3aR, fused to the maltose binding protein as antigen.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2A1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | C3AR1; C3AR; C3a-R; Complement component receptor 1; C3a anaphylatoxin chemotactic receptor |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length chimeric human antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds the 2e loop of the human C3a receptor, amino acid residues 185-199 and 218–226 (TNDHPWTVP). |
| Application Notes: | The original scFv antibody binds to the 2e loop of the human C3a receptor in an ELISA. Human C3a receptor is a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release. This antibody was able to bind RBL-2H3 cells stably expressing the C3aR and to the human mast cell line HMC-1 in a flow cytometric assay. This antibody is not capable of blocking the interaction between C3a and C3aR (PMID: 11373092). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |