Vector Labs Ab02824-3.0 Anti-Glycoprotein [MR78], Mouse IgG2b, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: MR78
Synonyms: GP; GP1; Envelope glycoprotein; GP1 2; Virion spike glycoprotein; Filovirus
Amount: 200 μg
Application Codes Clone: ELISA; neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody recognizes a GP1 epitope conserved across the filovirus family, which likely represents the binding site of their NPC1 receptor. This antibody also blocks binding of the NPC1 domain C to MARV GP. This antibody was also capable of reacting with Ebola virus (EBOV) GPcl that lacked the glycan cap and mucin-like domain (PMID: 25723164).
Application Notes: The binding specificity of the hybridoma supernatants for MARV glycoprotein was done using ELISA. ELISA was also performed to check whether the original Fab version of this antibody cross reacted with Ebola virus GP receptor-binding site. This antibody was also found to neutralize vesicular stomatitis virus/Marburg glycoprotein recombinant protein (VSV/GP-Uganda) and MARV-Uganda in a neutralization assay (PMID: 25723164). The crystal structure of this antibody in combination with Marburg virus Gp and Ebola virus GP demonstrates that the MR78 antibody recognizes a similar site on both MARV and EBOV. The epitope is a highly conserved hydrophobic trough revealed at the top of the EBOV GP1 core, after removal of the glycan cap by proteolytic cleavage in the endosome (PMID: 25723165). This antibody was recombinantly produced in tobacco (N. benthamiana) and was used in the in vivo therapeutic treatment of Marburg and Ravn virus infection in nonhuman primates like guinea pigs. This antibody was reported to provide 100% protection and virus could not be detected in plasma sampled 7 dpi in animals treated with MR78-N (PMID: 28381540). MR78 fails to neutralize VSVs bearing GPCL derived from any species other than MARV (PMID: 26908579).

Product Class:	Purified
Clone ID:	MR78
Synonyms:	GP; GP1; Envelope glycoprotein; GP1 2; Virion spike glycoprotein; Filovirus
Amount:	200 μg
Application Codes Clone:	ELISA; neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody recognizes a GP1 epitope conserved across the filovirus family, which likely represents the binding site of their NPC1 receptor. This antibody also blocks binding of the NPC1 domain C to MARV GP. This antibody was also capable of reacting with Ebola virus (EBOV) GPcl that lacked the glycan cap and mucin-like domain (PMID: 25723164).
Application Notes:	The binding specificity of the hybridoma supernatants for MARV glycoprotein was done using ELISA. ELISA was also performed to check whether the original Fab version of this antibody cross reacted with Ebola virus GP receptor-binding site. This antibody was also found to neutralize vesicular stomatitis virus/Marburg glycoprotein recombinant protein (VSV/GP-Uganda) and MARV-Uganda in a neutralization assay (PMID: 25723164). The crystal structure of this antibody in combination with Marburg virus Gp and Ebola virus GP demonstrates that the MR78 antibody recognizes a similar site on both MARV and EBOV. The epitope is a highly conserved hydrophobic trough revealed at the top of the EBOV GP1 core, after removal of the glycan cap by proteolytic cleavage in the endosome (PMID: 25723165). This antibody was recombinantly produced in tobacco (N. benthamiana) and was used in the in vivo therapeutic treatment of Marburg and Ravn virus infection in nonhuman primates like guinea pigs. This antibody was reported to provide 100% protection and virus could not be detected in plasma sampled 7 dpi in animals treated with MR78-N (PMID: 28381540). MR78 fails to neutralize VSVs bearing GPCL derived from any species other than MARV (PMID: 26908579).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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