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Mfg Part Number

Ab02826-3.0

Vector Labs Ab02826-3.0 Anti-YFV-E protein [7A], Mouse IgG2b, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated from two antibody-phage libraries constructed by repertoire cloning from yellow fever patients and subsequent panning with glycerol-purified non-inactivated YFV-17D virions.

$517.40

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Description

Product Class: Purified
Clone ID: 7A
Synonyms: E protein; Envelope protein E; Genome polyprotein
Amount: 200 μg
Application Codes Clone: ELISA; IP; neutralize; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds a conformational epitope on the envelope protein of the yellow fever virus comprising aa 153–155 in domain I and aa 71 in domain II of the E protein contributing to the epitope.
Application Notes: The original antibody showed 50% and 100% neutralizing activity in plaque reduction neutralization assay (PRNT) against YFV strains 17D-204-WHO and Asibi at concentrations of approximately 1 μg/ml and 10 μg/ml respectively. The original antibody neutralized wild-type YFV strains of genotypes West Africa I (Nigeria 1987) and II (Asibi) and East/Central Africa (CAR 1986, Ethiopia 1961) with comparable efficiency in a PRNT on PS cells. In a competition ELISA using biotinylated scFv-7A, this antibody fragment competed for binding to YFV-17D virions with the other neutralizing antibody fragments. The original scFv version of this antibody was able to immunoprecipitate a 55-kDa protein, corresponding to the molecular weight of the envelope glycoprotein of YFV-17D virions (PMID: 15919103).

Product Class:	Purified
Clone ID:	7A
Synonyms:	E protein; Envelope protein E; Genome polyprotein
Amount:	200 μg
Application Codes Clone:	ELISA; IP; neutralize; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds a conformational epitope on the envelope protein of the yellow fever virus comprising aa 153–155 in domain I and aa 71 in domain II of the E protein contributing to the epitope.
Application Notes:	The original antibody showed 50% and 100% neutralizing activity in plaque reduction neutralization assay (PRNT) against YFV strains 17D-204-WHO and Asibi at concentrations of approximately 1 μg/ml and 10 μg/ml respectively. The original antibody neutralized wild-type YFV strains of genotypes West Africa I (Nigeria 1987) and II (Asibi) and East/Central Africa (CAR 1986, Ethiopia 1961) with comparable efficiency in a PRNT on PS cells. In a competition ELISA using biotinylated scFv-7A, this antibody fragment competed for binding to YFV-17D virions with the other neutralizing antibody fragments. The original scFv version of this antibody was able to immunoprecipitate a 55-kDa protein, corresponding to the molecular weight of the envelope glycoprotein of YFV-17D virions (PMID: 15919103).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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