Vector Labs Ab02829-23.0 Anti-Shiga-like toxin 1 [13C4], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 13C4
Synonyms: SLT1; stxB; SLT-1 B subunit; SLT-1b; SLT-Ib; Shiga-like toxin 1 subunit B; Verocytotoxin 1 subunit B; Verotoxin 1 subunit B
Amount: 200 μg
Application Codes Clone: IP; neutralize; ELISA; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for the B subunit of Shiga-like toxin 1. This antibody does not bind B polypeptide of Stx2. Stx 1 is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Application Notes: The original IgG1 antibody neutralized the cytotoxicity of E. coli Shiga-like toxin (SLT) and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The original IgG1 antibody neutralizes Shiga toxin from Shigella dysenteriae type 1 and Shiga-like toxic activities in crude cell extracts from Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus, and Salmonella typhimurium. The IgG1 version of this antibody was also used in a colony blot assay for the detection of bacterial colonies which produce high levels of SLT (PMID: 3905611). This antibody neutralizes the cytotoxic and lethal activities of Stx1, This antibody was also reported to block the binding of Stx1 to its receptor, globotriaosyl ceramide by binding three nonlinear regions of the molecule that span receptor recognition sites on StxB1, one of which includes the essential residue 55N. This antibody was able to identify histidine-tagged StxB1 in a western blot assay. It did not recognize Stx1d (PMID: 17030576). This antibody was used in a sandwich ELISA as a detector antibody to identify production of Stx1 by E. coli strain MHI 813 (PMID: 12734256).

Product Class:	Purified
Clone ID:	13C4
Synonyms:	SLT1; stxB; SLT-1 B subunit; SLT-1b; SLT-Ib; Shiga-like toxin 1 subunit B; Verocytotoxin 1 subunit B; Verotoxin 1 subunit B
Amount:	200 μg
Application Codes Clone:	IP; neutralize; ELISA; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for the B subunit of Shiga-like toxin 1. This antibody does not bind B polypeptide of Stx2. Stx 1 is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Application Notes:	The original IgG1 antibody neutralized the cytotoxicity of E. coli Shiga-like toxin (SLT) and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The original IgG1 antibody neutralizes Shiga toxin from Shigella dysenteriae type 1 and Shiga-like toxic activities in crude cell extracts from Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus, and Salmonella typhimurium. The IgG1 version of this antibody was also used in a colony blot assay for the detection of bacterial colonies which produce high levels of SLT (PMID: 3905611). This antibody neutralizes the cytotoxic and lethal activities of Stx1, This antibody was also reported to block the binding of Stx1 to its receptor, globotriaosyl ceramide by binding three nonlinear regions of the molecule that span receptor recognition sites on StxB1, one of which includes the essential residue 55N. This antibody was able to identify histidine-tagged StxB1 in a western blot assay. It did not recognize Stx1d (PMID: 17030576). This antibody was used in a sandwich ELISA as a detector antibody to identify production of Stx1 by E. coli strain MHI 813 (PMID: 12734256).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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