Vector Labs Ab02830-23.0 Anti-Shiga-toxin 2 [11E10], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 11E10
Synonyms: SLT2; stxA2; stx2A; SLT-2 A subunit; SLT-2a; SLT-IIa; Shiga-like toxin 2 subunit A; Verocytotoxin 2 subunit A; Verotoxin 2 subunit A; rRNA N-glycosidase 2
Amount: 200 μg
Application Codes Clone: ELISA; IP; WB; neutralize; immunotherapy
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds a conformational epitope on the A subunit of Shiga-like toxin 2 comprising region A (StxA2 residues 42 to 49), region B (StxA2 residues 96 to 100), and region C (StxA2 residues 244 to 259) (PMID: 19433543). This antibody does not bind A subunit of Stx1. StxII is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Application Notes: The binding characterization of the original IgG1 version of this antibody towards Shiga like toxin 2 was done using ELISA. The original IgG1 version of this antibody was also used to immunoprecipitate SLT-Il toxoid. This antibody is capable of neutralizing the cytotoxicity of SLT-ll but not of SLT-I, Shiga toxin, or a variant of SLT-II produced by E. coli that causes edema disease of swine (PMID: 3053764). This antibody was also used a primary antibody in the detection of SLT-II in a western blot assay. It was reported that binding of 11E10 to Stx2 prevented the toxin from inhibiting protein synthesis in an in vitro assay but also altered the overall cellular distribution of Stx2 in Vero cells. It was suggested that binding of antibody 11E10 to Stx2 neutralizes the effects of the toxin by preventing the toxin from reaching and/or inactivating the ribosomes (PMID: 19433543). Administration of original mouse version of this antibody in a hemolytic-uremic syndrome (HUS) mouse model resulted in full recovery of mice after 4 days and the mice displayed normal renal function and normal levels of neutrophils and lymphocytes. These mice showed decreased fibrin(ogen) deposition and expression of proinflammatory mediators compared to those of Stx2-treated mice in the absence of antibody. It was concluded that passive immunization of mice with anti-Stx2 antibody prevented the lethal effects of Stx2 (PMID: 18694970). Another study reported that mice orally intoxicated with the original mouse version of this antibody got full protection and that orally intoxicated mice could be rescued by antibody 11E10 6 h but not 24 h after Stx2a delivery (PMID: 24379294).

Product Class:	Purified
Clone ID:	11E10
Synonyms:	SLT2; stxA2; stx2A; SLT-2 A subunit; SLT-2a; SLT-IIa; Shiga-like toxin 2 subunit A; Verocytotoxin 2 subunit A; Verotoxin 2 subunit A; rRNA N-glycosidase 2
Amount:	200 μg
Application Codes Clone:	ELISA; IP; WB; neutralize; immunotherapy
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds a conformational epitope on the A subunit of Shiga-like toxin 2 comprising region A (StxA2 residues 42 to 49), region B (StxA2 residues 96 to 100), and region C (StxA2 residues 244 to 259) (PMID: 19433543). This antibody does not bind A subunit of Stx1. StxII is a toxin produced by Shiga toxin (Stx)-producing E. coli (STEC) strains. Infections with STEC can lead to diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS).
Application Notes:	The binding characterization of the original IgG1 version of this antibody towards Shiga like toxin 2 was done using ELISA. The original IgG1 version of this antibody was also used to immunoprecipitate SLT-Il toxoid. This antibody is capable of neutralizing the cytotoxicity of SLT-ll but not of SLT-I, Shiga toxin, or a variant of SLT-II produced by E. coli that causes edema disease of swine (PMID: 3053764). This antibody was also used a primary antibody in the detection of SLT-II in a western blot assay. It was reported that binding of 11E10 to Stx2 prevented the toxin from inhibiting protein synthesis in an in vitro assay but also altered the overall cellular distribution of Stx2 in Vero cells. It was suggested that binding of antibody 11E10 to Stx2 neutralizes the effects of the toxin by preventing the toxin from reaching and/or inactivating the ribosomes (PMID: 19433543). Administration of original mouse version of this antibody in a hemolytic-uremic syndrome (HUS) mouse model resulted in full recovery of mice after 4 days and the mice displayed normal renal function and normal levels of neutrophils and lymphocytes. These mice showed decreased fibrin(ogen) deposition and expression of proinflammatory mediators compared to those of Stx2-treated mice in the absence of antibody. It was concluded that passive immunization of mice with anti-Stx2 antibody prevented the lethal effects of Stx2 (PMID: 18694970). Another study reported that mice orally intoxicated with the original mouse version of this antibody got full protection and that orally intoxicated mice could be rescued by antibody 11E10 6 h but not 24 h after Stx2a delivery (PMID: 24379294).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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