Vector Labs Ab02834-10.3 Anti-CD99 [12E7], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 12E7
Heavy Chain Modification: Fc Silent™
Synonyms: CD99 antigen; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; 12E7; MIC2; MIC2X; MIC2Y
Amount: 200 μg
Application Codes Clone: IF; RIA; WB; ICC; IHC; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds human CD99. CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. It plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane.
Application Notes: The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewing's sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). This antibody was also used in a study relating to the cell surface expression MIC2 antigen on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting (PMID: 7506950).

Product Class:	Purified
Clone ID:	12E7
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD99 antigen; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; 12E7; MIC2; MIC2X; MIC2Y
Amount:	200 μg
Application Codes Clone:	IF; RIA; WB; ICC; IHC; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds human CD99. CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. It plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane.
Application Notes:	The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewing's sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). This antibody was also used in a study relating to the cell surface expression MIC2 antigen on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting (PMID: 7506950).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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