Vector Labs Ab02839-23.0 Anti-VEGF-D [VD1 (4A5)], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: VD1 (4A5)
Synonyms: VEGFD; FIGF; Vascular endothelial growth factor D; c-Fos-induced growth factor
Amount: 200 μg
Application Codes Clone: Block; ELISA; IP; WB; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds human VEGF-D and block the binding of VEGF-D to both VEGFR-2 and VEGFR-3. Peptide-based mapping and site-directed mutagenesis suggested that binding epitope of this antibody includes the five residues 147NEESL151of the VEGF-D (PMID: 21315726). VEGF-D signaling through the VEGFR-3 receptor has been shown to be the primary source of lymphangiogenic activation and has also been noted in pathogenic angiogenesis in some tumors.
Application Notes: The original mouse antibody VD1 competes with VEGF-D to bind both VEGFR-2 and VEGFR-3 . VD1 blocks the mitogenic response of human microvascular endothelial cells to VEGF-D. The binding specificity of the original mouse version of this antibody towards human VEGF-D was determined using ELISA. The original mouse antibody was capable of immunoprecipitating human VEGF-D. This antibody was also capable of detecting VEGF-D in a western blot assay (PMID: 10785369). This antibody was used in a study involving a mouse tumor model to explore the role of VEGF-D in promoting both angiogenesis and lymphangiogenesis in tumors (PMID: 11175849). This antibody was also used to identify the role of VEGF-D in promoting tumor growth and spread though lymphatic system in a mouse model of hepatocellular carcinoma (PMID: 18338756). This antibody was used to study the localization of VEGF-D in primary tumors by immunohistochemical analysis (PMID: 12148568).

Product Class:	Purified
Clone ID:	VD1 (4A5)
Synonyms:	VEGFD; FIGF; Vascular endothelial growth factor D; c-Fos-induced growth factor
Amount:	200 μg
Application Codes Clone:	Block; ELISA; IP; WB; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds human VEGF-D and block the binding of VEGF-D to both VEGFR-2 and VEGFR-3. Peptide-based mapping and site-directed mutagenesis suggested that binding epitope of this antibody includes the five residues 147NEESL151of the VEGF-D (PMID: 21315726). VEGF-D signaling through the VEGFR-3 receptor has been shown to be the primary source of lymphangiogenic activation and has also been noted in pathogenic angiogenesis in some tumors.
Application Notes:	The original mouse antibody VD1 competes with VEGF-D to bind both VEGFR-2 and VEGFR-3 . VD1 blocks the mitogenic response of human microvascular endothelial cells to VEGF-D. The binding specificity of the original mouse version of this antibody towards human VEGF-D was determined using ELISA. The original mouse antibody was capable of immunoprecipitating human VEGF-D. This antibody was also capable of detecting VEGF-D in a western blot assay (PMID: 10785369). This antibody was used in a study involving a mouse tumor model to explore the role of VEGF-D in promoting both angiogenesis and lymphangiogenesis in tumors (PMID: 11175849). This antibody was also used to identify the role of VEGF-D in promoting tumor growth and spread though lymphatic system in a mouse model of hepatocellular carcinoma (PMID: 18338756). This antibody was used to study the localization of VEGF-D in primary tumors by immunohistochemical analysis (PMID: 12148568).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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