Vector Labs Ab02881-3.0 Anti-CD49E [SAM1], Mouse IgG2b, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: SAM1
Synonyms: SAM-1; Integrin Alpha 5
Amount: 200 μg
Application Codes Clone: FC; IF; IHC; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for human CD49E. CD49E is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. CD49E binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Application Notes: To determine the potential of human antibodies for use in an endocap-based approach, immunohistochemistry was used on snap-frozen samples of normal liver, hepatocellular cancer, liver metastases of pancreatic cancer, normal pancreas and primary pancreatic cancer using the mouse version of this antibody. Furthermore, the cytotoxic properties of this antibody were determined in a resazurin viability assay on HUVECs and two different tumour cell lines (Wang et al, 2019; pmid:31398471). To evaluate which ligands on the neutrophil mediate adhesion to VCAM-1, flow cytometry was performed on human neutrophils using the mouse version of this antibody (Lomakina and Waugh, 2009; pmid:19134480). To determine the interaction of cells with titanium, immunofluorescence was used on human osteoblastic cells with the mouse version of this antibody. Furthermore, this antibody was used for flow cytometry on MG-63 cells (Lüthen et al, 2005; pmid:15585246). To study how megakaryocytes differentiate and release platelets in the blood, immunofluorescence was performed on megakaryocytes using the mouse version of this antibody (Malara et al, 2011; pmid:21131589). To determine the regulatory effect of interleukin-4 (IL-4) on the expression and function of lymphocyte adhesion receptors involved in IL-2-induced cell aggregation, flow cytometry on peripheral blood lymphocytes using the mouse version of this antibody was performed. Furthermore, peripheral blood lymphocytes were used for immunofluorescence using this antibody (Vyth-Dreese et al, 1993; pmid:8473014).

Product Class:	Purified
Clone ID:	SAM1
Synonyms:	SAM-1; Integrin Alpha 5
Amount:	200 μg
Application Codes Clone:	FC; IF; IHC; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for human CD49E. CD49E is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. CD49E binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Application Notes:	To determine the potential of human antibodies for use in an endocap-based approach, immunohistochemistry was used on snap-frozen samples of normal liver, hepatocellular cancer, liver metastases of pancreatic cancer, normal pancreas and primary pancreatic cancer using the mouse version of this antibody. Furthermore, the cytotoxic properties of this antibody were determined in a resazurin viability assay on HUVECs and two different tumour cell lines (Wang et al, 2019; pmid:31398471). To evaluate which ligands on the neutrophil mediate adhesion to VCAM-1, flow cytometry was performed on human neutrophils using the mouse version of this antibody (Lomakina and Waugh, 2009; pmid:19134480). To determine the interaction of cells with titanium, immunofluorescence was used on human osteoblastic cells with the mouse version of this antibody. Furthermore, this antibody was used for flow cytometry on MG-63 cells (Lüthen et al, 2005; pmid:15585246). To study how megakaryocytes differentiate and release platelets in the blood, immunofluorescence was performed on megakaryocytes using the mouse version of this antibody (Malara et al, 2011; pmid:21131589). To determine the regulatory effect of interleukin-4 (IL-4) on the expression and function of lymphocyte adhesion receptors involved in IL-2-induced cell aggregation, flow cytometry on peripheral blood lymphocytes using the mouse version of this antibody was performed. Furthermore, peripheral blood lymphocytes were used for immunofluorescence using this antibody (Vyth-Dreese et al, 1993; pmid:8473014).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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