Vector Labs Ab02885-10.3 Anti-CD104 [58XB4], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 58XB4
Heavy Chain Modification: Fc Silent™
Synonyms: Integrin beta-4; ITGB4; GP150
Amount: 200 μg
Application Codes Clone: IHC; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for human CD104. Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.
Application Notes: To investigate the recruitment of plectin into hemidesmosomes by the α6β4 integrin, immunohistochemistry was performed on human MD-EBS keratinocytes using the mouse version of this antibody (Geerts et al, 1999; pmid:10525545). While showing that a subpopulation of cells in a hiPSC-derived corneal epithelial cell sheet is positive for ABCB5, flow cytometry was performed on human hiPSC-derived SEAMs using the mouse version of this antibody (Watanabe et al, 2021; pmid:34195566). The mouse version of this antibody was used in flow cytometry on differentiated human iPS cells to demonstrate the generation of a self-formed ectodermal autonomous multi-zone (SEAM) of ocular cells using human induced pluripotent stem cells (Hayashi et al, 2016; pmid:26958835). To study the intracellular accumulation of S. aureus in the epidermis of DFUs, flow cytometry was performed on single-cell suspensions from full-thickness DFU tissue from type 2 diabetes patients and control skin using the mouse version of this antibody (Pastar et al, 2021; pmid:34730110).

Product Class:	Purified
Clone ID:	58XB4
Heavy Chain Modification:	Fc Silent™
Synonyms:	Integrin beta-4; ITGB4; GP150
Amount:	200 μg
Application Codes Clone:	IHC; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for human CD104. Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.
Application Notes:	To investigate the recruitment of plectin into hemidesmosomes by the α6β4 integrin, immunohistochemistry was performed on human MD-EBS keratinocytes using the mouse version of this antibody (Geerts et al, 1999; pmid:10525545). While showing that a subpopulation of cells in a hiPSC-derived corneal epithelial cell sheet is positive for ABCB5, flow cytometry was performed on human hiPSC-derived SEAMs using the mouse version of this antibody (Watanabe et al, 2021; pmid:34195566). The mouse version of this antibody was used in flow cytometry on differentiated human iPS cells to demonstrate the generation of a self-formed ectodermal autonomous multi-zone (SEAM) of ocular cells using human induced pluripotent stem cells (Hayashi et al, 2016; pmid:26958835). To study the intracellular accumulation of S. aureus in the epidermis of DFUs, flow cytometry was performed on single-cell suspensions from full-thickness DFU tissue from type 2 diabetes patients and control skin using the mouse version of this antibody (Pastar et al, 2021; pmid:34730110).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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