Vector Labs Ab02886-10.3 Anti-CD227 [175C5], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 175C5
Heavy Chain Modification: Fc Silent™
Synonyms: Mucin-1; MUC1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin
Amount: 200 μg
Application Codes Clone: IHC; WB; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for human CD227. The alpha subunit of CD227 has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Application Notes: To characterize 13 different ovarian cancer xenografts, immunohistochemistry was performed different human ovarian cancer cell lines using the mouse version of this antibody. It only stained most tissues moderately (Molthoff et al, 1991; pmid:1985883). To determine whether the appearance of tumour-associated macin epitopes can be explained by altered post translational modification of mucin in tumours, flow cytometry was used on EBY-immortalized B-cells and Burkitt's lymphomas using the mouse version of this antibody (Jerome et al, 1992; pmid:1382849). To study three antigenic systems for their use as tumour toxins, a western blot was performed on the human mammary carcinoma cell line MBMDA-23 1 and the colon carcinoma cell line SW 1222 using the mouse version of this antibody (Wick and Groner, 1997; pmid:9459206).

Product Class:	Purified
Clone ID:	175C5
Heavy Chain Modification:	Fc Silent™
Synonyms:	Mucin-1; MUC1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin
Amount:	200 μg
Application Codes Clone:	IHC; WB; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for human CD227. The alpha subunit of CD227 has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Application Notes:	To characterize 13 different ovarian cancer xenografts, immunohistochemistry was performed different human ovarian cancer cell lines using the mouse version of this antibody. It only stained most tissues moderately (Molthoff et al, 1991; pmid:1985883). To determine whether the appearance of tumour-associated macin epitopes can be explained by altered post translational modification of mucin in tumours, flow cytometry was used on EBY-immortalized B-cells and Burkitt's lymphomas using the mouse version of this antibody (Jerome et al, 1992; pmid:1382849). To study three antigenic systems for their use as tumour toxins, a western blot was performed on the human mammary carcinoma cell line MBMDA-23 1 and the colon carcinoma cell line SW 1222 using the mouse version of this antibody (Wick and Groner, 1997; pmid:9459206).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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