Vector Labs Ab02888-23.0 Anti-CD11b [BEAR1], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: BEAR1
Synonyms: Complement Receptor 3; CR3; BEAR-1; Integrin alpha-M; ITGAM; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor
Amount: 200 μg
Application Codes Clone: functional assay; FC; IF; blocking
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for human CB11b. CB11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. CD11b is also identical with CR-3, the receptor for the iC3b fragment of the third complement component.
Application Notes: To investigate the role of adhesion molecules in the release of histamine, a histamine release assay was performed on human basophils under influence of the mouse version of this antibody (Watanabe et al, 1992; pmid:1384863). To study the expression of complement proteins on the surface of T lymphocytes, flow cytometry was performed on human T cells using the mouse version of this antibody (Wagner et al, 2001; pmid:11298342). To determine whether PMN ADCC also would be increased by the addition of an antibody/GM-CSF fusion protein and whether this would be associated with the up-regulation and activation of Mac-1, flow cytometry was performed on LA-N-1 neuroblastoma cells using the mouse version of this antibody (Metelitsa et al, 2002; pmid:12010822). Flow cytometry was performed on human full blood to create a standardized test for CD11b expression in PMN cells (Latger-Cannard et al, 2004; pmid:15163315). To study the uptake of L. pneumophila in monocytes using complement proteins, flow cytometry was used on MM6 cells using the mouse version of this antibody (Weissgerber et al, 2003; pmid:12620617). Immunofluorescence on isolated PMN was performed using the mouse version of this antibody to understand the action of surface bound elastase on PMN (Zimmermann et al, 2005; pmid:15718918). To study the interaction between CR3 and phagocytosis, an interaction experiment was performed between CD16 and PBMCs using the mouse version of this antibody as a blocking antibody. Furthermore, this antibody was used to immunophenotype PBMCs (Preynat-Seauve et al, 2004; pmid:14971040).

Product Class:	Purified
Clone ID:	BEAR1
Synonyms:	Complement Receptor 3; CR3; BEAR-1; Integrin alpha-M; ITGAM; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor
Amount:	200 μg
Application Codes Clone:	functional assay; FC; IF; blocking
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for human CB11b. CB11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. CD11b is also identical with CR-3, the receptor for the iC3b fragment of the third complement component.
Application Notes:	To investigate the role of adhesion molecules in the release of histamine, a histamine release assay was performed on human basophils under influence of the mouse version of this antibody (Watanabe et al, 1992; pmid:1384863). To study the expression of complement proteins on the surface of T lymphocytes, flow cytometry was performed on human T cells using the mouse version of this antibody (Wagner et al, 2001; pmid:11298342). To determine whether PMN ADCC also would be increased by the addition of an antibody/GM-CSF fusion protein and whether this would be associated with the up-regulation and activation of Mac-1, flow cytometry was performed on LA-N-1 neuroblastoma cells using the mouse version of this antibody (Metelitsa et al, 2002; pmid:12010822). Flow cytometry was performed on human full blood to create a standardized test for CD11b expression in PMN cells (Latger-Cannard et al, 2004; pmid:15163315). To study the uptake of L. pneumophila in monocytes using complement proteins, flow cytometry was used on MM6 cells using the mouse version of this antibody (Weissgerber et al, 2003; pmid:12620617). Immunofluorescence on isolated PMN was performed using the mouse version of this antibody to understand the action of surface bound elastase on PMN (Zimmermann et al, 2005; pmid:15718918). To study the interaction between CR3 and phagocytosis, an interaction experiment was performed between CD16 and PBMCs using the mouse version of this antibody as a blocking antibody. Furthermore, this antibody was used to immunophenotype PBMCs (Preynat-Seauve et al, 2004; pmid:14971040).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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