Vector Labs Ab02891-10.0 Anti-CD11a [SPVL1], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: SPVL1
Synonyms: SPV-L1; Integrin alpha-L; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain
Amount: 200 μg
Application Codes Clone: IP; functional assay; blocking; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for human CD11a. CD11a is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, F11R, and the secreted form of ubiquitin-like protein ISG15. CD11a is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. It contributes to natural killer cell cytotoxicity.
Application Notes: To prove that LFA-1 and Mo-1 have a similar backbone, immunoprecipitation was performed on human leukocytes using the mouse version of this antibody (Keizer et al, 1985; pmid:2933266). To characterize this antibody, immunoprecipitation was performed on JY cells using the mouse version of this antibody. Furthermore, a cytotoxic assay was performed on human CTL clones HG-38 (T3+, T4+) and HG-31 (T3+, T8) using this antibody. Finally, blood leukocytes, thymocytes, and several lymphoid cell lines were stained using this antibody (Spits et al, 1983; pmid:6332061). A proliferation assay was used on lymphocyte populations with the mouse version of this antibody to study autocrine proliferation in human helper T cell clones. The antibody only blocked proliferation stimulated by PBMC, but not by B-LCL (Pawelec et al, 1989; pmid:2521616).

Product Class:	Purified
Clone ID:	SPVL1
Synonyms:	SPV-L1; Integrin alpha-L; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain
Amount:	200 μg
Application Codes Clone:	IP; functional assay; blocking; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for human CD11a. CD11a is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, F11R, and the secreted form of ubiquitin-like protein ISG15. CD11a is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. It contributes to natural killer cell cytotoxicity.
Application Notes:	To prove that LFA-1 and Mo-1 have a similar backbone, immunoprecipitation was performed on human leukocytes using the mouse version of this antibody (Keizer et al, 1985; pmid:2933266). To characterize this antibody, immunoprecipitation was performed on JY cells using the mouse version of this antibody. Furthermore, a cytotoxic assay was performed on human CTL clones HG-38 (T3+, T4+) and HG-31 (T3+, T8) using this antibody. Finally, blood leukocytes, thymocytes, and several lymphoid cell lines were stained using this antibody (Spits et al, 1983; pmid:6332061). A proliferation assay was used on lymphocyte populations with the mouse version of this antibody to study autocrine proliferation in human helper T cell clones. The antibody only blocked proliferation stimulated by PBMC, but not by B-LCL (Pawelec et al, 1989; pmid:2521616).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews